Identification of a Luteovirus Associated with Rose Spring Dwarf Disease in Roses

Authors: N. Salem, A. Rowhani, A.L. Moyne, S.T. Sim, D.A. Golino
Keywords: RSD, dsRNA, Rosa multiflora, Luteoviridae
Abstract:
Double-stranded RNA (dsRNA) was extracted from roses that showed rose spring dwarf (RSD) disease symptoms on Rosa multiflora indicator host and negative disease symptoms on Shirofugen cherry. The dsRNA was used as a template for making a cDNA library. A virus which was closely related to members of the family Luteoviridae was identified. The virus was found to have five open reading frames (ORFs), similar to the genomic organization of luteoviruses. Similarities were observed in different coding regions for RNA-dependent RNA polymerase (ORF 1 and 2), coat protein (ORF 3), movement protein (ORF 4), and readthrough protein (ORF 5). A specific reverse-transcription polymerase chain reaction (RT-PCR)-based detection method was developed. We are currently in the process of characterizing this virus.

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